Journal: Carcinogenesis

Article Title: Development and molecular characterization of HCT-116 cell lines resistant to the tumor promoter and multiple stress-inducer, deoxycholate.

doi: 10.1093/carcin/23.12.2063

Figure Lengend Snippet: Fig. 11. Effect of two different antisense oligonucleotides, one targeted at the ATG codon (Oligo #1) and another 3 of the ATG codon selected on the basis of better secondary probe characteristics (Oligo #9) on DOC-induced apoptosis in resistant cell line HCT-116 RC.

Article Snippet: After 24 or 48 h of treatment with the antisense oligonucleotide, cells were fixed and stained with an antibody to p65 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) to determine the amount of reduction in protein expression.

Techniques:

Journal: Carcinogenesis

Article Title: Development and molecular characterization of HCT-116 cell lines resistant to the tumor promoter and multiple stress-inducer, deoxycholate.

doi: 10.1093/carcin/23.12.2063

Figure Lengend Snippet: Fig. 12. Down-regulation of NF-κB (p65) protein level in HCT-116RC cells by an antisense oligonucleotide. (A, B) Confocal images of cells stained with an antibody to p65 protein (red). Treatment with an antisense oligonucleotide (A) shows a decrease in p65 protein levels when compared with untreated control (B). Green fluorescence (C) indicates uptake of the p65 antisense oligonucleotides. (D) Immunocontrol for panels A and B, in which the primary antibody was omitted; minimal background fluorescence is shown.

Article Snippet: After 24 or 48 h of treatment with the antisense oligonucleotide, cells were fixed and stained with an antibody to p65 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) to determine the amount of reduction in protein expression.

Techniques: Staining, Control

Journal: Carcinogenesis

Article Title: Development and molecular characterization of HCT-116 cell lines resistant to the tumor promoter and multiple stress-inducer, deoxycholate.

doi: 10.1093/carcin/23.12.2063

Figure Lengend Snippet: Fig. 13. Effects of missense and antisense p65 oligonucleotides (probe #9) on DOC-induced apoptosis of cells of resistant cell line HCT-116RC. The secondary structure of the missense probe was designed to match that of the antisense probe #9.

Article Snippet: After 24 or 48 h of treatment with the antisense oligonucleotide, cells were fixed and stained with an antibody to p65 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) to determine the amount of reduction in protein expression.

Techniques:

Journal: Cell Death & Disease

Article Title: RP1-59D14.5 triggers autophagy and represses tumorigenesis and progression of prostate cancer via activation of the Hippo signaling pathway

doi: 10.1038/s41419-022-04865-y

Figure Lengend Snippet: A FISH assays determined the subcellular location of RP1-59D14.5 in PCa cells. Scale bar = 10 μm. B The starBase prediction of putative miRNAs of LATS1/2. Venn diagram illustrated a common miRNA of RP1-59D14.5 and LATS1/2. C RIP assays were performed to detect the association of RP1-59D14.5, miR-147a, and LATS1/2 with Ago2-RISC. D The sequence of miR-147a-Wt (wild type miR-147a) and miR-147a-Mut (mutant miR-147a) were shown. RNA pull down assays detected the enrichment of RP1-59D14.5 and LATS1/2. E RT-qPCR examined miR-147a expression in PCa cells transfected with miR-147a mimics. F Luciferase reporter assays detected the luciferase activity of wild-type or mutant-type RP1-59D14.5 and LATS1/2. ** P < 0.01.

Article Snippet: Biotinylated RP1-59D14.5 and wild-type or mutant-type biotinylated miR-147a (bio-miR-147a-Wt or bio-miR-147a-Mut) were synthesized by RiboBio (Guangzhou, China).

Techniques: Sequencing, Mutagenesis, Quantitative RT-PCR, Expressing, Transfection, Luciferase, Activity Assay

Journal: Cell Death & Disease

Article Title: RP1-59D14.5 triggers autophagy and represses tumorigenesis and progression of prostate cancer via activation of the Hippo signaling pathway

doi: 10.1038/s41419-022-04865-y

Figure Lengend Snippet: RP1-59D14.5 targets miR-147a/LATS1/2 axis as a ceRNA or recruits HUR to upregulate CK1 which could phosphorylate YAP and then recruit βTrCP to promote YAP degradation, consequently activating the Hippo signaling pathway in PCa cell.

Article Snippet: Biotinylated RP1-59D14.5 and wild-type or mutant-type biotinylated miR-147a (bio-miR-147a-Wt or bio-miR-147a-Mut) were synthesized by RiboBio (Guangzhou, China).

Techniques: